Nona Mordyukova was born in the village of Konstantinovskaya of the Donetsk region in Ukraine. In 1950 she graduated from the State Institute of Cinema and began working in a theatrical studio. In 1949 she had received the State Award of the USSR for her role of Ulyana Gromova in a film titled "The Young Guard" based on a novel of the same name by Soviet writer Alexander Fadeyev. In 1973 Mordyukova won the State Award of the Vasilyev brothers for her roles in films "Zhuravushka" and "The Collective Farm Director".
Her most outstanding roles were in films "Yekaterina Voronina", "The Volunteers", "Balzaminov's Marriage", "The Kinsfolk", "Mother", etc.
The editorial board of the British encyclopedia "Who is Who?" has included Mordyukova in the list of most outstanding actresses of the 20th century.